Electrochemiluminescence of an iridium complex doped with SiO2 nanoparticles to detect 2-adamantanamine based on the host-guest interaction of cucurbit[7]uril.

Abstract

The poor solubility of iridium complexes severely hampers its application in the electrochemiluminescence (ECL) sensing field. The doping of an iridium complex with silica (SiO2) nanospheres provides feasible solution for this problem. Herein, one kind of water insoluble iridium complex ([Ir(dFppy)2(d(CF3)bpy)](PF6)) was doped with SiO2 nanoparticles to obtain a luminescent nanocomposite (SiO2@Ir) which could generate strong ECL signals to act as a beacon molecule. An "off-on-off" mode ECL sensor was proposed based on the competitive host-guest interactions between 2-adamantanamine (2-ADA), ferrocene (Fc) and cucurbit[7]uril (Q[7]). Fc could be used as an inhibitor to decrease the ECL signal, while Q[7] could wrap Fc to recover the ECL signal. Q[7] has a stronger binding ability with 2-ADA than Fc. As a result, 2-ADA could replace Fc from the cavity of Q[7], and the ECL signal was inhibited again. Under the optimal conditions, the ECL intensity varied linearly with 2-ADA concentration in the range of 1.0 × 10-8 to 1.0 × 10-6 mol L-1 with a detection limit of 2.5 × 10-10 mol L-1. The results not only provided a new avenue for the application of water insoluble iridium complexes in ECL sensing, but also revealed the attractive potential of host-guest interactions in the fabrication of biosensors.