Abstract
Electrochemiluminescence (ECL) is a light-emitting phenomenon triggered by electrochemistry. The resulting optical signal enables spatiotemporal resolution of the luminescent signal that can be imaged with high accuracy. The field of ECL is evolving rapidly from an analytical method widely applied in biosensing to a novel microscopy technique. The efficient generation of ECL depends on the electrode materials, the luminophore/coreactant systems, and experimental configurations, focusing research on improving these parameters. Nowadays, ECL enables the analysis of transient events and micro/nanometric objects, surface boundaries, interfaces, and physical phenomena, as well as catalytic and biological processes. Since ECL microscopy offers many novel applications, this article will describe first the context and then the most recent advances in ECL imaging of single objects and living cells or subcellular structures.
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