Electrochemiluminescence Biosensor for Nucleolin Imaging in a Single Tumor Cell Combined with Synergetic Therapy of Tumor.

Abstract

Nucleolin, a nuclear biological multifunctional protein, plays significant roles in modulating the proliferation, survival, and apoptosis of tumor cells. Different from the traditional electrochemiluminescence (ECL) method, a new ECL biosensor was built to perform ECL imaging of nucleolin in a single HeLa cell with high sensitivity and throughput. Briefly, mesoporous silica nanoparticles (MSN) loaded with doxorubicin (DOX) and phorbol 12-myristate 13-acetate (PMA) were used as drug carriers and could be specifically opened by nucleolin in a HeLa cell. PMA then induced the HeLa cell to produce reactive oxygen species (ROS) and realized ECL imaging of nucleolin. After that, ROS could damage DNA and proteins of the tumor cell and DOX could induce the apoptosis of HeLa cells by inhibiting genetic material, nucleic acid, synthesis. HeLa cells were then efficiently killed by DOX and ROS in a synergetic pathway. Herein, a new ECL biosensor for ECL imaging of nucleolin in a single HeLa cell and synergetic tumor therapy was built.