Abstract
Electrochemically oxidized ferrocene and eleven ferrocene derivatives were investigated as catalysts for the chemiluminescent oxidation of luminol by hydrogen peroxide. The catalytic effect has been related to the redox potential of the ferrocenes. The effect of twelve different buffers with different pKa values and four different electrode materials was also investigated. A combination of ferrocenedicarboxylic acid, bis tris propane buffer and an ITO electrode gave the most intense light emission and eliminated measurable electrogenerated background chemiluminescence. Phenol labelled with ferrocenemonocarboxylic acid via a peptide bond catalyzed more intense chemiluminescence than the parent ferrocene derivative. Results were used to suggest a structure for ferrocene labelled antigens that can be used in competitive immunoassays.
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