Electrochemically Deposited Porous Graphene−Polypyrrole−Polyphenol Oxidase for Dopamine Biosensor

Abstract

AbstractWe report a method for the fabrication of glassy carbon electrode modified porous graphene‐polypyrrole‐polyphenol oxidase (GCE−PG−PPy−PPO) modified electrode for the determination dopamine. The optimization of pH, concentration and detection limit of dopamine was employed by amperomatric technique. The detection limit of dopamine was found to be in a linear range of 2×10−8 to 4.6×10−5 M and lower limit detection is 4×10−9 M. Michealis – Menten constant (Km) and the activation energy were calculated as 31.32 μM and 37.4−Kj mol−1, respectively. The developed biosensor was used to quantify the dopamine in human urine sample.