Abstract

Zinc oxide nanoparticles (ZnONPs), multiwalled carbon nanotubes (MWCNTs) and 1,4-benzoquinone (BQ) dispersed in chitosan (CS) matrix were used to construct a xanthine biosensor. Xanthine oxidase (XOx) was immobilized onto BQ-MWCNTs-ZnO–CS composite modified glassy carbon electrode (GCE) using glutaraldehyde as the crosslinking agent. The parameters of the construction process and the experimental variables for the biosensor were optimized. The xanthine biosensor showed optimum response within 10 s, and the sensitivity was 39.4 μA/mMcm 2 at +0.25 V (vs. Ag/AgCl). The linear working range of the biosensor was found to be 9.0×10 −7 - 1.1×10 −4 M with a detection limit of 2.1×10 -7 M. The biosensor exhibited good long-term stability and reproducibility. The presented biosensor was also used for monitoring the freshnesses of chicken and beef flesh.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.