Abstract
An electrochemical voltammogram recording method for plant variety identification is proposed. Electrochemical voltammograms of Vistula, Andromeda, Danuta, Armandii ‘Apple Blossom,’ Proteus, Hagley Hybrid, Violet Elizabeth, Kiri Te Kanawa, Regina, and Veronica’s Choice were recorded using leaf extracts with two solvents under buffer solutions. The voltametric data recorded under different conditions were derived as scatter plots, 2D density patterns, and hot maps for variety identification. In addition, the voltametric data were further used for genetic relationship studies. The dendrogram deduced from the voltammograms was used as evidence for relationship study. The dendrogram deduced from voltametric data suggested the Andromeda, Danuta, Proteus, Regina, and Hagley Hybrid were closely related, while Violet Elizabeth and Veronica’s Choice were closely related. In addition, Vistula and Armandii ‘Apple Blossom’ could be considered outliers among the varieties.
Highlights
The typical morphological features of ornamental plants are specific, visually visible external features
The electrochemical reaction was taken at the surface of the GCE using a differential pulse voltammetry (DPV) scan
The recorded voltammograms of Clematis were submitted for pattern generation
Summary
The typical morphological features of ornamental plants are specific, visually visible external features They can directly reflect the evolutionary course and genetic relationship of plants. They can be used as the main bases for variety identification and classification. Though comparative morphological methods are relatively simple and intuitive [1,2], plant morphology is affected by environmental conditions and by gene expression [3,4]. A large sample size is a prerequisite for cytological identification and classification. Molecular markers have the advantages of a high stability, good repeatability, and wide distribution in the genome [23,24] They are affected by factors such as sample size, the number of markers, and polymorphism. It is difficult to ensure the reliability of results when using a particular method for variety identification
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