Electrochemical Test Strip-Based Accurate Blood Uric Acid Measurement by Adding Blood Cell Filtration Membrane

Abstract

In this paper, we demonstrated a novel electrochemical uric acid (UA) test strip by integrating the blood cells filtration membrane with the electrochemical UA test strip. The conventional electrochemical UA biosensor suffered from the effect of blood cells in the whole blood sample. The blood cells in the whole blood can impede the UA molecules transfer from the bulky blood solution to the surface of the enzyme-immobilized working electrode. If the concentration of the blood cells was extremely higher/lower, the electrochemical current signal generated by the electrochemical sensor was significantly prohibited, which was capable of resulting in the wrong measured result. The fatal drawbacks of the conventional UA test strip limited its application in blood UA monitoring at the point of care scale. In the proposed structure of the new designed electrochemical UA test strip, the blood cells filtration membrane was added in the blood collection pore. The blood plasma was driven by the hydrophilic force to fill the electrochemical reaction channel. The effect of the blood cells on the measured result can be significantly prohibited. The clinical verification of the proposed UA test strip was evaluated by comparing to the bulky biochemical analyzer. The experimental results demonstrated that the proposed UA test strips were precise enough with comparable accuracy to the clinical laboratory standard, which is a very promising tool for precisely monitoring the blood UA.