Abstract
The interaction of levofloxacin, a fluoroquinolone antimicrobial drug, with double-stranded calf thymus DNA was studied in solution by cyclic voltammetry at glassy carbon electrode. Voltammetric behavior of levofloxacin was also studied on dsDNA-modified electrodes. The binding of levofloxacin with DNA, immobilized onto the surface of an anodically activated carbon paste electrode, was exploited for designing a sensitive biosensor for levofloxacin. The response was optimized with respect to accumulation time, ionic strength, drug concentration, reproducibility and other variables. A linear dependence of the peak currents on the concentration was observed in the range of 5.0×10 −7 to 5.0×10 −6 M, with a detection limit of 1.0×10 −7 M. Direct and simple determination of levofloxacin in urine was established with no manipulation of urine sample other than dilution. The detection limit of the method was 25 μg ml −1 of levofloxacin in urine. The method was validated using HPLC as a reference method.
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