Electrochemical sensor for secretolytic agent-ambroxol at eriochrome black -T modified carbon electrode

Abstract

A novel electrochemical sensor for the determination of secretolytic agent ambroxol was constructed by electrochemical deposition of eriochrome black-T (EBT) on surface of carbon paste electrode (CPE). Different voltammetric techniques like cyclic voltammetry, linear sweep voltammetry and square wave voltammetry were used. Modified paste electrode was characterized by atomic force microscope (AFM). The voltammetry dependence on current, potential, pH, concentrations, scan rate, temperature and excipients were investigated in optimized experimental conditions. According to the liner relation between peak potential, peak current, scan rate, effect of temperature, heterogeneous rate constant ambroxol concentration, differential pulse voltammetric was used for the quantitative determination in phosphate buffer solution. The linear response was obtained in the range of with a detection limit LOD = 4.8 nM. The possible electrochemical oxidation of two electrons and two proton free radical mechanisms proposed and activation parameters of ambroxol were determined. Further, the sensor was successfully applied in pharmaceutical and biological fluid sample analysis.