Electrochemical sensing of label free DNA hybridization related to breast cancer 1 gene at disposable sensor platforms modified with single walled carbon nanotubes

Abstract

The electrochemical monitoring of DNA hybridization related to specific sequences on breast cancer 1 (BRCA1) DNA by using singlewalled carbon nanotube (SWCNT) based screen printed graphite electrodes (SPEs) was performed in this study. After the microscopic characterization of SWCNT-SPE, the optimization of assay was studied. The development of screen printing process combined with nanomaterial based disposable sensor technology with sensitivity, selectivity and reproducibility, has a great opportunity for DNA detection using differential pulse voltammetry (DPV) by measuring the guanine oxidation signal observed at +1.00V in the presence of DNA hybridization between BRCA1 probe and its complementary target. The detection limit estimated for signal to noise ratio=3 corresponds to 378.52nM target concentration in the 40μL samples. The voltammetric results on BRCA1 DNA hybridization were also complemented with electrochemical impedance spectroscopy (EIS).