Abstract
Graphene is an advanced material. We confirmed in our previous paper that graphene is very sensitive to biofilm formation. Therefore, it may be utilized as a sensor for biofilm formation in the future. In this experiment, we used graphite and multi-layer graphene, which we obtained by repeating an exfoliation method. The biofilm formation was evaluated by crystal violet staining, Raman spectroscopy, and chronoamperometry in dilute sulfuric acid. The final technique was an adventurous trial. However, the method provided us with reasonable data, as compared with the results of the other two conventional methods.
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