Abstract
Abstract An efficient and scalable NAD(P)+ regeneration system to promote alcohol dehydrogenase-catalysed oxidation reactions is reported. Indirect electrochemical oxidation of NADH was established with 2,2′-azino-bis-(3 ethyl-benzo-thiazoline-6-sulfonic acid) (ABTS), being the most efficient mediator amongst the candidates screened. ABTS exhibited very high catalytic performance of 1200 catalytic turnovers per hour. In a three-dimensional electrochemical cell with a high working electrode surface area of 24 m2 and optimised concentrations of substrate, enzyme, cofactor and mediator, TTNs of 1860 for the mediator and of 93 for the cofactor were measured. Besides, a maximum STY of 1.4 g l−1 h−1 was determined. Here we show the highest TTN ever reported for a mediated NAD+ regeneration in an electro-enzymatic process. The use of the three-dimensional electrochemical reactor led to an 8-fold improvement of the STY compared to a published system, based on a two-dimensional cell.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
