Electrochemical reduction and voltammetric determination of diloxanide furoate in non-aqueous medium

Abstract

The electrochemical reduction of diloxanide furoate (DF) in acetonitrile on glassy carbon electrode was studied in this work. It was observed that DF is reduced after a reversible one-electron transfer followed by an irreversible chemical reaction, diagnosed as C-Cl bond cleavage. Its reduction was followed by linear (LSV), differential pulse (DPV) and square wave voltammetry (SWV). Analytical curves were obtained for DF determination using all the investigated voltammetric techniques. For LSV was obtained a linear range (LR) from 5.0 x 10-4 to 1.0 x 10-2 mol L-1, with detection limit (DL) of 1.5 x 10-4 mol L-1 and sensitivity (S) of 2.1 x 10(4) µA mol-1 L. The analytical parameters obtained by DPV were: LR = 5.0 x 10-4 to 2.2 x 10-3 mol L-1, DL = 7.8 x 10-5 mol L-1, S = 3.7 x 10(4) µA mol-1 L. For SWV were obtained a LR = 7.5 x 10-6 to 1.2 x 10-3 mol L-1, DL = 5.5 x 10-6 mol L-1 and S = 2.8 x 10(5) µA mol-1 L. Thus, the SWV was the most sensible technique, which can be used for DF determination at low concentration levels. Statistics methods were used to evaluate the analytical procedure, where recovery around to 100% was obtained for all voltammetric techniques. Relative standard deviations were lower than 5.0% (N=5). The obtained t values evaluating all the three voltammetric methods were less than the tabulated ones, indicating that there are no evidences of systematic error.