Abstract
Reduced nicotinamide adenine dinucleotide (NADH) was oxidized coulometrically at a rotating platinum gauze electrode at 0.7 V vs. SCE at pH 9. The NAD + formed was reduced enzymatically in a reactor containing immobilized alcohol dehydrogenase. Several recyclings were made with the same lot of NADH. The coulometric results were in reasonable agreement with spectrophotometric measurements. The overall conversion to enzymatically active NAD + showed a current efficiency of 99.3%. The electrode pretreatment described is essential for high current efficiency. A continuous method of oxidation of alcohol with electrolytic regeneration of cofactor and removal of aldehyde by dialysis was tested.
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