Electrochemical Oxidation and Determination of Antiviral Drug Acyclovir by Modified Carbon Paste Electrode With Magnetic CdO Nanoparticles.

Ebrahim Naghian,Hamid Reza Pazoki-Toroudi,Ali Sobhani-Nasab,Elnaz Marzi Khosrowshahi,Farhad Ahmadi,Mehdi Rahimi-Nasrabadi,Esmail Sohouli

doi:10.3389/fchem.2020.00689

Abstract

With the development of nanomaterials in electrochemical sensors, the use of nanostructures to modify the electrode surface has been shown to improve the kinetics of the electron transfer process. In this study, a sensor was developed for the electrochemical determination of Acyclovir (ACV) based on the modified carbon paste electrode (CPE) by CdO/Fe3O4. The magnetic CdO nanoparticles characterization was studied by energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD). To study of the modified CPE surface morphology, scanning electron microscopy (SEM) was used. At the optimal conditions, a noteworthy enhancement in the electrochemical behavior of ACV was observed at the surface of the modified CPE compared to the unmodified CPE. A detection limit of 300 nM and a linear range of 1–100 μM were obtained for the quantitative monitoring of ACV at the modified CPE surface using differential pulse voltammetry (DPV) in phosphate buffer. The RSD% (relative standard deviation) of the electrode response was <4.3% indicating the development of a high precision method. Also, satisfactory results were obtained in the determination of ACV with the modified electrode in tablet, blood serum, and urine samples with a satisfactory relative recovery (RR%) in the range of 94.0–104.4%.

Highlights

Herpes simplex is a type of skin infection caused by the herpes hominies virus (Braun-Falco et al, 1991) and is commonly known as herpes blister
The first phase is the Fe3O4 sample shows a series of diffraction peaks at the position of 35.45◦ [(311) line], 43.08◦ [(400) line], 57.16◦ [(511) line], and 62.72◦ [(440) line] that is in good agreement with the standard JCPDS file of Fe3O4 Cubic phase and CdO shows a series of diffraction peaks at the position of 33.35◦ [(111) line], 38.78◦ [(200) line], 55.52◦ [(220) line], 65.92◦ [(311) line], and 69.33◦ [(222) line] which is in good agreement with the standard JCPDS with the crystal structure of tetragonal (JCPDS 750592) with a space group of Fd-3m, respectively
The results showed that from 3 to 10 incremental trends are observed in the signal, but after 7% this trend continues with a lower slope, so for optimal consumption of nanoparticles, this value was used as the optimal percentage in the structure of the electrode

Summary

INTRODUCTION

Herpes simplex is a type of skin infection caused by the herpes hominies virus (Braun-Falco et al, 1991) and is commonly known as herpes blister. ACV is widely used in the therapy of herpes zoster infections, herpes simplex, herpetic encephalitis, primary genital herpes, and varicella-zoster virus infections in immunosuppressed patients It is helpful in inhibiting HSV infections in renal allograft receptors (Tenser and Tenser, 2019) and its anti-hepatitis B virus activity has been demonstrated (Huang et al, 2011). Its analysis in human serum is complicated and requires high selective analytical methods With notice of these facts, the quantitative monitoring of ACV in biological fluids and other real samples appears very important. The Fe3O4 magnetic nanoparticles were synthesis by a reaction between 0.02 mol of iron (II) chloride and 0.03 mol of iron (III) chloride These salts were dissolved in a 40 mL of degassed HCl (0.4 M) solution and 375 mL of degassed ammonia solution (25%) was added to the above solution during 45 min drop by drop under sonication. The ACV content of samples was measured using the standard addition method

RESULTS AND DISCUSSION

CONCLUSIONS

ETHICS STATEMENT