Abstract
Interaction of uranyl ion (UO22+) with immobilized double strand calf thymus DNA (ds-ct-DNA) on zirconium attached gold-mercaptopropionic acid self-assembled monolayer (Au–MPA–Zr(IV) SAM) is monitored by electrochemical techniques. The results show that after 15min proximity of the immobilized DNA with UO22+, the peak currents of the square wave voltammograms are decreased (about 80%), and the equivalent circuit model of electrochemical impedance spectroscopy (EIS) data, requires two constant phase elements (CPE) instead of only one. By using the surface concentration of DNA (≈2.3×10−13mol/cm2) and the number of the anthraquinonedisulfonic acid (AQDS) adsorbed on DNA (1.34×10−10mol/cm2) the ration of AQDS per DNA base pairs is obtained ≈1/30 before proximity to UO22+. Based on EIS technique, we find that the double strand structure of immobilized DNA on the electrode surface has been changed (damaged) by UO22+. This modified electrode has potential of becoming a screening tool for the rapid assessment of the interaction and genotoxicity of existing and new chemicals.
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