Electrochemical Monitoring of Interaction of Temozolamide with DNA by Graphene Oxide Modified Single-Use Electrodes

Abstract

Graphene oxide (GO) modified single-use pencil graphite electrodes (PGEs) were used for electrochemical monitoring of surface confined interaction between Temozolamide (TMZ) and calf thymus double stranded DNA (ctdsDNA). First, the PGE surface was activated by EDC/NHS (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide) as covalent agents (CA). Then, modification of GO was done onto the surface of chemically activated PGE surface to obtain CA-GO-PGEs. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy studies were used to establish the interfacial electron transfer of the electrodes. The optimization of experimental conditions was carried out via CV technique followed by electrochemical examination of the interaction of TMZ with ctdsDNA.