Abstract
We report on the proof-of-principle of an amperometric method to monitor the progress of a polymerase chain reaction (PCR) in real-time. It is based on the finding that the intercalating redox probe Methylene Blue (MB) becomes less easily electrochemically detectable once intercalated into double-stranded DNA (ds-DNA) compared to its free form. This was studied by cyclic voltammetry before and after addition of salmon sperm ds-DNA. Under optimized conditions, the products of the PCR of mitochondrial DNA fragments were quantitatively detected at different stages of amplification cycles. This strategy is potentially cheaper and easier to integrate into a hand-held miniaturized device than fluorescence-based real-time PCR.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
