Electrochemical investigation on the interaction of diclofenac with DNA and its application to the construction of a graphene-based biosensor

Abstract

We have investigated the interaction between diclofenac and deoxyribonucleic acid (DNA) by the electrochemical method. On glassy carbon electrode, the voltammetric curve of diclofenac showed an oxidation peak, which was obviously influenced by the addition of DNA. Accordingly, a series of electrochemical experiments were carried out to elucidate the interaction between diclofenac and DNA. Based on the diclofenac–DNA interaction, a biosensor for diclofenac was developed. When a film of DNA was immobilized on the electrode surface, DNA showed an oxidation signal promoted by graphene oxide. Due to the diclofenac–DNA interaction, the peak current of DNA decreased and the peak potential shifted positively with increasing the concentration of diclofenac. The response of the biosensor was linear to the concentration of diclofenac in the range of 1 to 130 μM. Using such a biosensor, the photodegradation of diclofenac was successfully monitored.