Abstract
Copper-plated screen-printed carbon electrode (CuSPE) provides a suitable catalytic surface for the amperometric detection of hydrogen peroxide. Glucose oxidase (GOD) is immobilized on the top of the CuSPE to form a glucose sensor. The interaction of copper oxide with GOD was found to be an important factor in the glucose detection. Preliminary investigation under hydrodynamic conditions showed a linear calibration plot up to 26.7 mM glucose with a slope and regression coefficient of 4.5 μA/mM and 0.9902, respectively. The Michaelis-Menten kinetics by nonlinear curve fitting yielded a Km value close to that in solution indicating the ideality and suitability of the present system. Classical mixed potential mechanism is for the first time applied to the enzyme-coated CuSPE to further understand the system.
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