Abstract
A novel electrochemical immunosensor for highly sensitive detection of deoxynivalenol (DON) in food samples was successfully developed based on single-walled carbon nanotubes/chitosan. The principle was based on the indirect competition between anti-DON, free DON and DON–bovine serum albumin on a glassy carbon electrode modified by the functionalized nanotube/chitosan films. Anti-rabbit immunoglobulin G secondary antibody labelled with alkaline phosphatase was used to react with primary antibody. Differential pulse voltammetry signal could be generated from the hydrolysis of substrate α-naphthyl phosphate catalysed by alkaline phosphatase. The electrochemical signal was linear with the logarithm of DON concentrations, ranging from 0.01 to 1000 ng mL−1 with a detection limit of 5 pg mL−1 under optimal conditions. In addition, the designed strategy was successfully applied to assay DON in different kinds of food samples, showing satisfactory recovery and consistency with high-performance liquid chromatography.
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