Abstract
An amperometric biosensor for the quantification of food allergens based on an inhibitory immunoassay is presented. As a proof of concept, the experimental conditions were optimized for the detection of β-casein in the 0-10 ppm range. Eight electrochemical cells were integrated into a small-sized portable potentiostat controlled by a smartphone via Bluetooth communication. The determination of β-casein in eight different samples can be measured with the electrochemical biosensor, which has the potential to be modified for the detection of multiple allergens.
Highlights
Food allergies are a worldwide growing concern due to its impact on food safety and public health
The determination of food allergens is a subject of increasing concern
The development of an amperometric electrochemical biosensor associated with an inhibitory immunoassay presented in this work for the detection of food allergens has several advantages
Summary
Food allergies are a worldwide growing concern due to its impact on food safety and public health. We present the preliminary results of an alternative method for the detection of β-casein in the order of a few ppm consisting in a portable immunosensor devised for the analysis of food allergens. It is based on antigen-antibody reactions between soluble and immobilized antigens. Carboxylic groups formed on the carbon electrode after plasma treatment were treated with 100 μl of 0.1 M EDC and 10 μl of 25 mM NHS for 30 minutes and, after rinsing, incubated for 2 hours with 50 μl of a 100 ppm β-casein solution in 0.1 M phosphate buffer of pH 7.0. When the optimized values found for ELISA were used in the electrochemical biosensor, it was found that higher antibody titers were necessary; a 1:10000 and 1:2000 dilutions were used for the primary and secondary antibodies, respectively
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