Abstract
Monitoring of circulating N-terminal proBNP (NT-proBNP) biomarkers is crucial for the diagnosis of people suffering from heart failure (HF). In this work, we describe a novel ultra-sensitive NT-proBNP immunosensor for NT-proBNP detection in artificial human saliva. The surface of the developed immunosensor based on gold working microelectrodes (WEs) was biofunctionalized through carboxyl diazonium to immobilize anti-NT-proBNP antibodies. The chemical surface modification of WEs was carried out by cyclic voltammetery CV whilst the quantification of NT-proBNP biomarkers was made by electrochemical impedance spectroscopy (EIS). The immunosensor has demonstrated a linear detection response within the range 1–20 pg/mL for NTproBNP detection in artificial human saliva with a good selectivity in the presence of other interferences.
Highlights
Heart failure (HF) is a clinical syndrome where in cardiac output is insufficient to meet the metabolic demands of the body
After thorough cleansing and functionalization, the surface of biosensor’s working microelectrodes (WEs) was characterized by cyclic voltammetry (CV) at 80 mV/s and the switching potential was scanned between −0.2 to 0.6 mV)
Fe2+/Fe3+ redox peaks have decreased after the CMA deposition
Summary
Heart failure (HF) is a clinical syndrome where in cardiac output is insufficient to meet the metabolic demands of the body. Existing methods of diagnosis for HF rely heavily on classical methods which are based on tests conducted in central laboratories that may take several hours or even days from when tests are ordered to when results are received [2] To solve this problem, biosensors can play an important role in the early diagnosis without having to rely on hospital visits where expensive and time-consuming laboratory tests are recommended [3] one of the most commonly used biomedical approach for detection heart failure is determining the level of valuable specific biomarkers which are generated in different stage. Proceedings 2018, 2, 1085 very interesting biological matrix which has high potential for the surveillance of general health and disease, potentially important biomarkers are increased in saliva during local and systemic inflammation These biomarkers are being detected through different techniques [4]. The immunosensor has been used in artificial saliva and has demonstrated good sensitivity
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