Abstract
A quantitative and sensitive immunochromatographic strip test is developed to detect of melamine in milk products. The strip test based on antigen-antibody (melamine-anti melamine) complex reaction. As label to antibody is used silver nanoparticles to form antibody-silver nanoparticles (AgNP-Ab). The strip test consists of sample pad, conjugated pad (AgNP-Ab), nitrocelloluse membrane, test zone (Ab), and absorbant pad. When melamine is found in the sample, then antibody-melamine-antibody-silver nanoparticles complex was made. Silver nanoparticles have good electrochemical properties. Electrochemical measurement of silver nanoparticles is using anodic stripping voltammetry method. The strip test electrode device is using Ag/AgCl as reference electrode, glassy carbon (GC) as working electrode and platinum (Pt) wire as counter electrode. The quantity of silver nanoparticles is involved in the system is equivalent to the concentration of melamine. Therefore, quantitative detection of melamine could be done using the immunochromatographic strip test.
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