Electrochemical Imaging for Single-cell Analysis of Cell Adhesion Using a Collagen-coated Large-scale Integration (LSI)-based Amperometric Device

Abstract

We here report the electrochemical imaging of cell adhesion using a large-scale integration (LSI)-based amperometric device, called a Bio-LSI device. The device consists of 400 sensor electrodes arranged with a pitch of 250 µm. The device surface was modified with collagen to assist in the culture of MCF-7 cells and promote their adhesion. The cells disturb the electrochemical reaction of redox mediators, allowing the electrochemical signal to be used to evaluate cell adhesion at the single-cell level. This approach was applied to a cell detachment test. The results show that the Bio-LSI device is a promising tool for single-cell analysis.