Abstract
In this study, an electrochemical biosensor composed of a horseradish peroxidase (HRP)-encapsulated protein nanoparticles (HEPNP) was fabricated for the sensitive and selective detection of H2O2. The HEPNP has a three-dimensional structure that can contain a large amount of HRP; therefore, HEPNP can amplify the electrochemical signals necessary for the detection of H2O2. Furthermore, reduced graphene oxide (rGO) was used to increase the efficiency of electron transfer from the HEPNP to an electrode, which could enhance the electrochemical signal. This biosensor showed a sensitive electrochemical performance for detection of H2O2 with signals in the range from 0.01–100 μM, and it could detect low concentrations up to 0.01 μM. Furthermore, this biosensor was operated against interferences from glucose, ascorbic acid, and uric acid. In addition, this fabricated H2O2 biosensor showed selective detection performance in human blood serum. Therefore, the proposed biosensor could promote the sensitive and selective detection of H2O2 in clinical applications.
Highlights
Hydrogen peroxide (H2O2) is a very important in the fields of chemistry, biology, food studies, and in applications in clinical studies [1,2,3]
It can be confirmed that H2O2 in the serum can be measured using a constant signal. These results demonstrate that the HRP-encapsulated protein nanoparticles (HEPNP)/reduced graphene oxide (GO) (rGO)/Gold electrode (Au)-based electrochemical biosensor can selectively detect H 2O2 in human serum with stable response and can be applied to clinical sample analysis
4 Conclusion In this study, an electrochemical biosensor composed of HEPNP was fabricated for highly sensitive and selective H2O2 detection
Summary
Hydrogen peroxide (H2O2) is a very important in the fields of chemistry, biology, food studies, and in applications in clinical studies [1,2,3]. 3.3 Electrochemical properties of HEPNP/rGO/Au biosensor for sensitive detection of H2O2 To confirm the electrochemical signal enhancement of the fabricated HEPNP biosensor in the detection of H2O2, the different modified electrodes were prepared respectively in the presence of 100 μM H 2O2 in 0.01 M PBS solution.
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