Abstract
BRCA1 is the biomarker for the early diagnosis of breast cancer. Detection of BRCA1 has great significance for the genetic analysis, early diagnosis and clinical treatment of breast cancer. In this work, we developed a simple electrochemical DNA sensor based on a DNA tetrahedral-structured probe (TSP) and poly-adenine (polyA) mediated gold nanoparticles (AuNPs) for the sensitive detection of BRCA1. A thiol-modified TSP was used as the scaffold on the surface of the screen-printed AuNPs electrode. The capture DNA (TSP) and reporter DNA were hybridized to the target DNA (BRCA1), respectively, to form the typical sandwich system. The nanocomposites of reporter DNA (polyA at the 5′ end) combined with AuNPs were employed for signal amplification which can capture multiple enzymes by the specificity between biotin and streptavidin. Measurements were completed in the electrochemical workstation by cyclic voltammetry and amperometry and we obtained the low limit of detection of 0.1 fM with the linear range from 1 fM to 1 nM. High sensitivity and good specificity of the proposed electrochemical DNA sensor showed potential applications in clinical early diagnosis for breast cancer.
Highlights
Breast cancer is one of the most common malignancies and the major leading cause of cancer death among females in over 100 countries [1]
We developed a simple electrochemical DNA sensor based on tetrahedral-structured probe (TSP) and polyA
The principle of the proposed electrochemical DNA sensor for BRCA1 detection was based on the electrochemical signals of the redox reaction in the presence of the TMB
Summary
Breast cancer is one of the most common malignancies and the major leading cause of cancer death among females in over 100 countries [1]. It is indispensable to diagnose breast cancer in the early stage. The breast cancer susceptibility gene (BRCA1) is a human tumor-suppressor gene that is involved in DNA damage repair [2]. The detection of BRCA1 is of great significance for the genetic analysis, early diagnosis and clinical treatment of breast cancer. The traditional detection methods of BRCA1 include single-strand conformation polymorphism assay (SSCP), high-performance liquid chromatography (HPLC) and DNA sequencing [5,6,7,8]. These methods have limitations in analytical time, cost and simplicity. It is necessary to develop a rapid, sensitive, low-cost and simple method for BRCA1 detection
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