Abstract
The application of electrochemical techniques for DNA detection is motivated by their potential to detect hybridization events in a more rapid, simplistic and cost-effective manner compared to conventional methods. Here, we present an electrochemical DNA biosensor for the specific and quantitative detection of single-stranded DNA (ssDNA). Probe oligonucleotides were immobilized onto gold electrodes by a 5′ end thiol-group linker. Following hybridization with the complementary DNA, the cobalt complex was electrochemically accumulated on the double-stranded DNA layer and the differential pulse voltammogram (DPV) for this electrode gave an electrochemical signal due to the redox reaction of [Co(phen)3]2+/3+ that was bound to the double-stranded DNA on the electrode, and it can sense single base-pair mismatche within the sequence of the hybridized double-stranded DNA (dsDNA).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
