Abstract
A novel electrochemical aptasensor, based on disposable screen-printed electrodes was developed for the sensitive detection of the mycotoxin ochratoxin A (OTA). Two strategies were investigated by using an indirect and a direct competitive assay, based on the use of superparamagnetic nanoparticles. The performance of the optimized aptasensors in terms of reproducibility, stability, sensitivity, and analysis time was studied. The best strategy was found to be the direct competitive format. In this assay, free OTA competed with labeled alkaline phosphatase–OTA for the binding to the DNA aptamer immobilized on magnetic beads. The electrochemical detection was thus achieved through a suitable substrate for the enzyme ALP, by Differential Pulse Voltammetry. The aptasensor obtained using this novel approach allowed detection limit of 0.11 ng/mL, and was also validated for real sample analysis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
