Electrochemical Determination of Uric Acid in Urine by Using Zeolite Imidazolate Framework-11 Modified Electrode

Tran Si Thanh,Tran Thanh Tam Toan,Le Van Thanh Son,Nguyen Thi Thanh Tu,Tran Thi Bich Hoa,Phan Tu Qui,Dinh Quang Khieu,Tran Ngoc Tuyen,Do Mai Nguyen,Thanh-Danh Nguyen

doi:10.1155/2021/9914062

Abstract

In the present article, the synthesis of zeolite imidazole framework-11 (ZIF-11) by ultrasonic-assisted hydrothermal process and its application as an electrode modifier for electrochemical determination of uric acid in urine are demonstrated. The obtained materials were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and nitrogen adsorption/desorption isotherms. It was found that the ZIF-11 with rhombic dodecahedron topology and high surface area (1066 m2.g-1) was synthesized in a certain temperature and found in around 25–40°C, and other crystalline phases of zinc benzimidazolate deferring from ZIF-11 phase were found in less 25°C or higher than 40°C. The ZIF-11 is stable in the pH range 6-10. The modification of glassy carbon electrode was performed with ZIF-67 using the drop-casting procedure. The present ZIF-11 modified electrode was employed to study the electrochemical behavior of uric acid (UA). UA oxidation is catalyzed by this electrode in aqueous buffer solution (pH 7) with a decrease of 70 mV in overpotential compared to glassy carbon electrode. With the differential pulse–anodic stripping voltammetry (DP-ASV) method, the oxidation current of UA versus its concentration shows good linearity in the range 20–540μM ( R = 0.998 ) with a detection limit of 0.48 μM ( S / N = 3 ). The obtained ZIF-11 modified electrode was applied in the detection of UA content in urine samples, and satisfied results were obtained.

Highlights

Uric acid (UA, C5H4N4O3) is a natural waste product from the digestion of foods that contain purines
These results indicate that zeolite imidazole framework-11 (ZIF-11)/GCE can be effectively employed for the uric acid (UA) determination in real biological samples with excellent concurrent results (Table 4)
ZIF-11 materials could be obtained in a temperature range of about 25–40°C

Summary

Introduction

Uric acid (UA, C5H4N4O3) is a natural waste product from the digestion of foods that contain purines. The detection of the UA with a facile method is clinically important because it serves as a marker for the determination of the mentioned diseases For this purpose, various techniques have been used, such as enzymatic system [1], calorimetry [2], fluorescence [3], high-performance liquid chromatography [4], gas chromatography-mass spectrometry [5], and voltammetry [6]. ZIF-11 with rhombic dodecahedron (RHO) topology exhibits larger cages connected through pore apertures and possesses unusual thermal and chemical stability in water and organic solvents [13] Thanks to these exceptional properties, ZIF-11 is one of the most promising ZIFs [13]. The electrochemical determination of uric acid on a glassy carbon electrode modified with ZIF-11 is demonstrated

Experimental

Result and Discussion

Findings

Voltammetric Determination of Uric Acid on ZIF-11Modified GCE

Conclusions