ELECTROCHEMICAL DETERMINATION OF THE INTERACTION BETWEEN ANTICANCER DRUG CAPECITABINE AND DNA BY CARBON PASTE ELECTRODE

Abstract

In this study, the interaction of an anticancer drug, Capecitabine with DNA was investigated by electrochemical methods using carbon paste electrode (CPE). The interaction of Capecitabine with single-stranded Calf thymus DNA (ss DNA) and double-stranded Calf thymus DNA (ds DNA) at electrode surface, or in solution phase was investigated by monitoring the changes at the oxidation signal of guanin base of DNA measured by differential pulse voltammetry (DPV) technique. Impedimetric measurements were performed by electrochemical impedance spectroscopy technique that also confirmed that ds DNA was immobilized onto the electrode surface. The detection limit (DL) in the case of interaction of Capecitabine with dsDNA and ssDNA interaction was calculated and found to be 17.35µg/mL and 17.12µg/mL, respectively.