Abstract
Present study aims to improve the modified Brdicka procedure of metallothionein (MT) quantification in a way to reduce the analysis duration. Therefore, scan rate of the voltammetric sweep was increased from 0.0052 V s–1 to 0.013 V s–1 by lowering the duration of one step potential from 0.5 s to 0.2 s, which resulted in 2.5 times faster voltammetric sweep. Research confirmed high accuracy and reliability of MT measurements, in both standard sample and samples of heat-treated cytosols of liver, gills and the intestine of European chub and liver of brown trout. The method was confirmed as fast and reliable electrochemical technique for quantification of MTs and this time- saving improvement is especially useful and applicable in different biomonitoring studies which require the analyses of numerous biological samples and high sample throughput.
Highlights
M ETALLOTHIONEINS (MTs) are the family of inducible metal-binding proteins which have important roles in the homeostasis of essential trace metals (Cu and Zn) and/or sequestration of toxic metals (e.g., Ag, Cd and Hg), as well as in the protection against oxidative damage.[1,2] They are characterized by low molecular mass, lack of aromatic amino acids and high cysteine content
Calibration curves were constructed using MT-2 rabbit liver standard for both parameter settings, previously used and explained by Raspor et al.[13] and novel with the modified instrumental measuring conditions applied in this study
Measurement, included the change in two measurement parameters, duration of one step potential was lowered from 0.5 s to 0.2 s, and scan rate was increased from 0.0052 V s–1 to 0.013 V s–1
Summary
M ETALLOTHIONEINS (MTs) are the family of inducible metal-binding proteins which have important roles in the homeostasis of essential trace metals (Cu and Zn) and/or sequestration of toxic metals (e.g., Ag, Cd and Hg), as well as in the protection against oxidative damage.[1,2] They are characterized by low molecular mass, lack of aromatic amino acids and high cysteine content. Increased MTs reduce toxic effects caused by metals and at cellular level are one of the first detectable signs of the occurrence of elevated metal levels. MTs are often used in biomonitoring studies as biomarkers of metal exposure.[2,3]
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