Electrochemical determination of chloramphenicol on glassy carbon electrode modified with multi-walled carbon nanotube–cetyltrimethylammonium bromide–poly(diphenylamine)

Abstract

A glassy carbon electrode (GCE) modified with multi-walled carbon nanotube–cetyltrimethylammonium bromide–poly(diphenylamine) (MWCNT–CTAB–PDPA) composite film was constructed and used to determine chloramphenicol (CAP) levels in biological samples. Diphenylamine (DPA) was successfully electropolymerized onto MWCNT–CTAB modified GCE using cyclic voltammetry in 1mM monomer solution and 5M H2SO4. The surface morphology of the MWCNT–CTAB–PDPA film was characterized using scanning electron microscopy and electrochemical impedance spectroscopy. The results showed that the electrochemical reduction of CAP on the modified electrode was adsorption-controlled. The effects of scan rate, electrolyte solution, accumulation condition, and amounts of MWCNT and CTAB were optimized. Under optimized conditions, the electrode showed a linear response in the range of 1.0×10−8–1.0×10−5M (R2=0.9997) and a detection limit of 2.0×10−9M. The sensor was successfully applied to determine the level of CAP in milk and honey samples.