Electrochemical determination of ATP at rhodamine6G capped gold nanoparticles modified carbon felt electrode at pH 7.2

Abstract

We report the electrochemical determination of adenosine-tri-phosphate (ATP) using rhodamine6G capped gold nanoparticles (FCGN) modified carbon felt (CF) as working electrode in phosphate buffer solution (PBS; 0.2 M) as a supporting electrolyte at pH 7.2. It was found that FCGN-modified-CF electrode not only enhanced the oxidation currents, but also showed uniform shift in the current with repetitive potential cycles. Selective determination of ATP in the presence of high concentration of AMP, ADP, H2PO4– and HP2O73– and the effect of pH on electrochemical behavior of ATP was also examined. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) studies demonstrate typical peaks ca. + 0.60 V and +2.25 (vs. SCE), respectively with excellent selectivity and sensitivity. The interface property of FCGN-modified-CF electrode was investigated by impedance spectroscopy. Under optimum experimental conditions, the calibration plots are linear in the 10–100 nM range with detection limit of (LoD) ∼ 8.0 nM. Furthermore, the sensitivity of Rh6 G–Au NPs towards ATP was reconfirmed by fluorescence titration study, which is in accord with electrochemical experimental findings.