Electrochemical detection of ornidazole in commercial milk and water samples using an electrode based on green synthesis of silver nanoparticles using cellulose separated from Phoenix dactylifera seed

Abstract

The widespread use of antibiotics has contributed to the control of disease and the nutritional well-being of livestock. Antibiotics reach the environment via excretions (urine and feces) from human and domestic animals, through non proper disposal or handling of unused drugs. The present study describes a green method for the synthesis of silver nanoparticle (AgNPs) using cellulose extracted from Phoenix dactylifera seed powder via mechanical stirrer method for the electroanalytical determination of ornidazole (ODZ) in milk and water samples. The cellulose extract is used as the reducing and stabilizer agent for the synthesis of AgNPs. The obtained AgNPs were characterized by UV–Vis, SEM and EDX, presenting a spherical shape and an average size of 48.6 nm. The electrochemical sensor (AgNPs/CPE) was fabricated by dipping a carbon paste electrode (CPE) in the AgNPs colloidal solution. The sensor shows acceptable linearity with ODZ concentration in the linear range from 1.0 × 10−5 to 1.0 × 10−3 M with a limit of detection (LOD =3S/P) and quantification (LOQ =10S/P) of 7.58 × 10−7 M and 2.08 × 10−6 M respectively.