Abstract
This paper reports a novel electrochemical method for the detection of insulin in ultra-pure water. A screen printed electrode (SPE) modified by Nickel hydroxide was used as the working electrode, one with good activity of electro-catalytic oxidation-reduction for insulin. The SPE was modified by Nickel hydroxide using the electrodeposition method. The results showed that Nickel hydroxide was homogeneously electrodeposited on the surface of the working electrode. A coin-size electrolytic tank was fabricated with PDMS, which only requires 0.3ml of sample for the detection. The concentration of insulin was analyzed using the methods of Cyclic voltammetry (CV), Electrochemical impedance spectroscopy (EIS) and Chronoamperometry (i-t). The Nickel hydroxide used for detecting insulin showed good analytical characteristics, such as a high sensitivity of $\pmb{14.797\mu} \mathbf{A}\bullet\pmb{\mu} \mathbf{M}^{\pmb{-1}}$ , a good linear detection range of $\pmb{100}\mathbf{nM}\pmb{-5\mu} \mathbf{M}$ , and a low detection limit of 93nM. This nickel hydroxide modified SPE electrode has a much smaller size and is easier to fabricate than traditional electrodes, making it a much more promising candidate for an insulin sensor.
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