Abstract
Hydrogen peroxide (H 2O 2) biosensors are some of the most promising sensors being developed, because they are highly selective and respond quickly to a specific substrate. Hemoglobin (Hb) has been used as an ideal biomolecule for the construction of hydrogen peroxide biosensors because of its high sensitivity to H 2O 2. Direct electron transfer from Hb has been widely investigated for application in the detection of H 2O 2 because of the reaction’s simplicity, high selectivity and intrinsic sensitivity. A method for the electrochemical detection of hydrogen peroxide based on the immobilization of hemoglobin on a DNA/Fe(pyterpy) 2+-modified gold electrode was investigated. The pyterpy monolayers were primary electron depositions on the gold electrode surface of a quartz crystal microbalance (QCM). This layer offers a template to which negatively charged DNA attaches. The process of fabricating the electrode was verified by a quartz crystal analyzer (QCA). The experimental parameters, such as pH, applied potential and amperometric response, were evaluated and optimized. Under the optimized conditions, this sensor shows a linear response between 3.0 × 10 −6 and 9.0 × 10 −4 M concentrations of H 2O 2. The detection limit was determined to be 7 × 10 −7 M (based on S/ N = 3).
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