Abstract
The present paper describes the use of methylene blue (MB) as an electroactive label on a pencil graphite (lead) electrode (PGE) to provide a well-defined recognition interface for the detection of HPV target DNA. In order to construct the sensor, a 20-mer single strand oligonucleotide probe related to human papilloma virus (HPV) major capsid protein L1 gene was immobilized on the PGE electrode. Hybridization event between the probe and its complementary sequence was studied by measurement of MB signal accumulated on the PGE using square wave voltammetry (SWV) method. Some hybridization experiments with noncomplementary oligonucleotides were carried out to examine the selectively of the sensor to the target DNA from other DNAs related to Hepatitis C virus (HCV), fungi, and bacterial genes. Moreover, some factors affecting the function of sensor including electrode activation and probe immobilization condition were also investigated. The data showed that the constructed electrode detects the target DNA with detection limit of 1.2 ng μl−1 and discriminates it from various DNAs originated from a wide variety of organisms.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
