Electrochemical Detection of Heparin Based on Its Interaction with Light Green

Abstract

AbstractThe determination of heparin with linear sweep voltammetry has been developed in this paper based on its interaction with light green (LG). In pH 2.0 Britton–Robinson (B–R) buffer solution, LG has a sensitive linear sweep voltammetric reduction peak at −0.41 V (vs. SCE) and heparin can react with LG to form an ion‐association electro‐active complex, which resulted in the decrease of the peak current of the reaction solution. Under the selected experimental conditions the decrease of peak current was directly proportional to the concentration of heparin in the range of 0.8–20.0 μg/mL with the linear regression equation as Δi$\rm{ {_{p}^{\prime\prime }}}$ (nA)=79.12 C−101.44 (n=14, γ =0.996) and the detection limit as 0.28 μg/mL (3σ). The effects of coexisting substances on the determination of heparin were investigated and the results showed that this method had good selectivity. The method was further applied to the determination of heparin content in heparin samples with satisfactory results and good recovery.