Abstract
NADH plays a vital role in the electron transfer processes between metabolites in the cellular energetic reactions. Therefore, there is a crucial need to develop analytical techniques for detecting NADH levels with the metabolism of glucose. In the present study, a nanocomposite of alumina (Al2O3) nanoparticles confined graphene oxide (GO) sheet acts as a modifier for carbon paste electrode (CPE) for a sensitive detection of NADH level in a mediator-less detection scheme. Our findings after optimization of experimental conditions reveal that, there is a remarkable enhancement in the direct electron transfer through the Al2O3-GO nanocomposite surface with high electrocatalytic activity towards NADH oxidation. Results show that, there is a linear increase in NADH detection from 30 µM to 330 µM, together with linear regression coefficient of 0.98 and LOD 4.5 µM. These results confirm that, the developed Al2O3-GO based CPE electrode is a promising electrode for real NADH level detection in practical enzymatic applicability.
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