Abstract
In this work, a noble metal based platinum (Pt) electrode has been explored to detect one of the important phytochemical Aloe Emodin (AE) in Liliaceae family. A sensitive three-electrode system was equipped to assess the electrochemical behavior of AE using Pt electrode as a working electrode along with silver/silver chloride (saturated KCl) and a steel as a reference electrode and a counter electrode, respectively. The redox response of Pt electrode against AE was analyzed using cyclic voltammetry (CV) at a scan rate ranging from 0.01 Vs-1 to 0.4 Vs-1 that showed well-defined reversible peaks in phosphate buffer saline (PBS at pH 6.0). Under optimized experimental condition, DPV peak currents were linear over concentration range 1 µM -150 µM with R2 value of 0.987 In addition, the sensitivity of the electrode was excellent with sensitivity factor of 1.3 A/M and the limit of detection (LOD) was obtained 0.2 µM. Principal component analysis (PCA) was performed on the response data to demonstrate diverse discrimination of different concentration of AE. The developed sensor showed excellent repeatability and can be successfully used to detect AE in Aloe.
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