Abstract
Electrochemical Characterization for Determination of PNA Surface Density and Rate Electron Transfer Constant Based on Methylene Blue Accumulation Charge
Highlights
Probe density is one of important factor in order to improve detection signal in electrochemical biosensor [1]
These method will determine the amount of methylene blue (MB) as redox molecule that was adsorbed into ssPNA was translated to the ssPNA surface density according to equation
TP= NA TMBΧZ / M ( Na) where ƬPNA is PNA surface density, Z is the charge of MB and M is the number of bases in PNA (5_-CTCGTCCAGCGCCGCTTCGG-3), NA is Avogadro’s number and Ƭ is MB surface coverage, where ƬMB value was calculated from Equation: Q = nFAT
Summary
Probe density is one of important factor in order to improve detection signal in electrochemical biosensor [1]. The use cross-linking and conjugation of method to immobilization of NH2-containing biomolecules onto carboxyl-containing substrates via covalent amide bond by using EDC and NHS most widely used to control over the immobilization of the probe oligonucleotides. The amount of EDC and NHS been used to bound amine biomolecule in previous study seem different between modified electrode. Equal amounts of EDC and NHS (100mM) were used for activation of carboxylic acid terminated self-assembled monolayers [4]. In this study, we shared result obtained of amount of 1-ethyl-(dimethylaminopropyl) carbodiimide hydrcholoride (EDC) and N-hydroxy succinimide (NHS) used as controlling agent to determine PNA probe density on SPCE modified graphene quantum dots nanomaterial once the carboxyl groups to produce NHSS-ester [5] (Figure 1). The rate of methylene blue (MB) been investigated
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