Electrochemical Biosensors Integrating Metallic and Non-Metallic Nanomaterials for Biomedical Applications

Abstract

In this talk, I will present our latest efforts made on creating highly sensitive and selective electrochemical biosensors integrating either carbon nanotubes or gold nanoparticles in addition to biological molecules or polyelectrolytes for medical diagnostic applications. Single or multi-walled carbon nanotubes and graphenes wrapped with polyelectrolytes were implemented onto screen printed carbon electrode (SPCE) surfaces for enhancing the sensors sensitivity. Whereas target analyte selective enzymes were utilized to improve the selectivity. The oppositely charged polyelectrolyte coated carbon nanomaterials and enzymes were immobilized on the electrode surface via a simple layer by layer assembly process. Our interest of analytes were hypoxanthine, xanthine, uric acid, dopamine and serotonin, which can be used for diagnosis of various diseases including cardiovascular disorders and game addiction syndrome. The developed biosensors were successfully applied to both normal and patient serum analyses. In addition, a brief introduction of utilizing target selective DNA aptamer coated gold nanoparticles deposited on SPCE for the selective voltammetric detection of protein tyrosine kinase-7, which is known to be a potential lung cancer biomarker. Finally, I will discuss future aspects and challenging issues of our nanomaterial integrated biosensing methodologies.