Abstract
Electrochemical biosensor is an effective tool for pharmaceutical analysis due to its simplicity, specificity, sensitivity, fast, cost effective and repetitive measurements with miniaturized and portable devices. The paper illustrates the detail methodology for development of an electrochemical biosensor based on hemoglobin (Hb) film modified for anticancer drug adriamycin (ADM). Square wave voltammetry (SWV) studies of the electrodes before and after immobilizing of HB shows the successful formation of a selectivity of the electrode. The proposed Hb based biosensor allows quantitation over the range 1 to 8010-8M, The suggested biosensor method can be successfully applied to the detection and determination of anticancer drug adriamycin in different drug formulations and can be suggested as a suitable sensor for analysis of Adriamycin in biological samples.ً.
Highlights
Cancer is a very serious health problem in all developed countries
Biological Effect of Adriamycin Kiyomia et al performed some experiments to identify the intracellular specificity of the differential cytotoxic effects of adriamycin on neoplastic and normal cells. These results suggest that adriamycin exerts cytostatic effects on neoplastic and normal undifferentiated cells through the inhibition of DNA synthesis by DNA intercalation, and cytotoxic effects on neoplastic cells through the accumulation of reactive oxygen species resulting from low scavenger enzyme activities
In the case of model solutions of ADM, The analyze was added with a micropipette to the supporting electrolyte consisting 0.2 M PBS (10.0 mL). the potential was swept in the range from -1000 mV to -200 mV vs. Ag/AgCl, the peak potential at (-601) mV under default conditions of the instrument (Fig 2) shows the square-wave voltammogram obtained for at pH 7 of a bare graphite electrode
Summary
Cancer is a very serious health problem in all developed countries. Since the beginning of the 21st century, cancer has becomes one of the most common primary causes of death, of which malignant breast tumors predominate for women (Risbridger et al, 2010; Cosimo and Baselga, 2010 ; Lin et al, 2010) and prostate cancer in men (Capitanio and Suardi, 2011; Lawrentschuk and Klotz, 2011; Oon et al, 2011). Voltammetric methods, based on the use of different working electrodes, have proven to be convenient for the determination of this drug, thanks to the fact that its structure contains electroactive groups (Vacek et al, 2009).
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