Electrochemical Behaviors and Electrochemical Determination of Tiamulin Fumarate at Acetylene Black-Ionic Liquid Modified Glassy Carbon Electrode

Abstract

Acetylene black-ionic liquid modified glassy carbon electrode(AB-ILs/GCE) was prepared by acetylene black Ionic liquid and characterized by electrochemical impedance spectroscopy(EIS).The electrochemical behaviors and electrochemical kinetics of tiamulin fumarate(TF) at AB-ILs/GCE were investigated by cyclic voltammetry(CV),chronoamperometry(CA) and Square Wave voltammetry(SWV).The experimental result showed an irreversible oxidation peak appeared on GCE at about 0.74V.However,the oxidation peak potentials of TF keep constant on AB/GCE than that of GCE,the oxidation peak currents on AB/GCE increased about 1.8 times than that of GCE;compared with AB/GCE,the oxidation peak potentials of TF on AB-ILs/GCE shifted 20mV negatively,the peak currents on AB-ILs/GCE increased 1.6 times than that of AB/GCE.The experimental result indicated that AB-ILs/GCE could catalyze the oxidation of TF well The electrochemical kinetic parameters were also determined on AB-ILs/GCE.The influence of experimental conditions was investigated.The quantitative determination of TF content was investigated,the relative standard deviation is between 1.1%-2.9% and the recoverage is in the range of 98.6%-101.8%.The method has been applied to the quantitative determination of TF content in injection sample.