Abstract
The use of advanced electrochemical methods provides a significant methodological approach to a variety of tasks and research problems in bioelectrochemistry. Of particular importance in terms of bioelectrochemical aspects is the electrochemical behavior of viruses at charged interfaces. The gold electrode is considered ideal for investigating the behavior of biomolecules in aqueous media. Gold nanoparticles (nAu) have unique properties that allow them to be used to facilitate electron transfer between the electrode surface and biomolecules, making them a promising choice as a bioelectrochemical catalyst. The aim of the study is the analysis and physicochemical characterization of the Newcastle virus (NCV) La Sota strain, Newvac vaccine in phosphate buffer solution (PB) using gold nanoparticles as sensitizers. With respect to the electrochemical and electrical parameters, the Au/electrolyte interface constituted a system studied by Open Circuit Potentiometry (OCP), Cyclic Voltammetry (CV), Electrochemical Impedance Spectroscopy (EIS), Square Wave Voltammetry (SWV) and Chronopotentiometry (CP) in association with UV-Vis spectrophotometry. Gold nanoparticles have the role of sensitizing the redox processes in which the La Sota virus participates, as indicated by the amount of charge passing through the interface associated with anodic processes (Qa / Qc) increases from 0.31 (PB), 0.43 (PB-nAu), 0.54 (PB_NCV) to 0.61 (PB_nAu_NCV).
Published Version
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