Abstract
This paper reports the selective determination of isoproterenol (IP) in the presence of uric acid (UA) and folic acid (FA) using 2,7-bis(ferrocenyl ethyl)fluoren-9-one modified carbon nanotube paste electrode (2,7-BFCNPE) in 0.1 M phosphate buffer solution (PBS) (pH 7.0). The bare carbon paste electrode does not separate the voltammetric signals of IP, UA, and FA. However, 2,7-BFCNPE not only resolved the voltammetric signals of IP, UA, and FA with potential differences of 150, 325, and 475 mV between IP–UA, UA–FA, and IP–FA, respectively, but also dramatically enhanced the oxidation peak currents of them when compared to bare carbon paste electrode. In PBS of pH 7.0, the oxidation current increased linearly with two concentration intervals of IP, one is 0.08 to 17.5 μM and the other is 17.50 to 700.0 μM. The detection limit (3σ) obtained by DPV was 26.0 ± 2 nM. The practical application of the modified electrode was demonstrated by determining IP in IP injection, urine, and human blood serum.
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