Abstract
Hypocrellin A (HA) is a natural perylene quinonoid drug, which can kill tumor cells efficiently, so, based on this theory, a graphene and HA modified glassy carbon electrode was fabricated in this paper, and the interaction between HA and telomere DNA was investigated in pH 6 phosphate buffer solution by electrochemical methods for the first time. Thermodynamic study indicated that the binding is mainly van der Waals and hydrogen bond. The fluorescence experiment showed that the binding ratio (n) of HA and telomere DNA were found to be 1.04 at 293 K, 0.71 at 298 K, 0.68 at 308 K, and 0.61 at 310 K, respectively. Under the optimized conditions, the oxidation current of HA was proportional to human telomere DNA concentration in the range from 5.91×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-9</sup> to 7.27×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-7</sup> mol/L, with the correlation coefficient of 0.9920 and the detection limit of 1.21×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-10</sup> mol/L (S/N = 3). The proposed method was further applied to determine DNA in blood serum and abdominal fluid samples with satisfactory results.
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