Abstract
Abstract Nitric oxide (NO) is an important, short-lived bio-regulatory messenger molecule that plays a critical part in a variety of biological functions. The selective and reliable detection of NO in biological systems is crucial in order to research and understand the role of NO. Non-electrochemical methods for the detection of NO have been reported but are not well suited for realtime measurement and are very time consuming. A few electrochemical NO sensors have also been described but have significant drawbacks in terms of both selectivity and sensitivity for NO. Moreover, these systems have been limited to researchers with a good understanding of electro-analytical theory and often require an external reference electrode, which can complicate and limit the experimental design (1, 2). Only recently has the technology become available to reliably and specifically measure NO in biological systems. World Precision Instruments (WPI) released recently the first commercially available NO electrode for general laboratory use. The system is described here and offers distinct advantages over those previously reported and enables quick, easy, and reliable NO measurement.
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